Benchtop RNA Purification from Fresh or Frozen Cells. The Maxwell® RSC simplyRNA Cells Kit, used with the Maxwell® RSC Instruments, provides a simple method for automated RNA purification from fresh or frozen cells. The low elution volume is used to generate concentrated high-quality RNA suitable for use in downstream applications such as 32-5/8" or 38-5/8" 3. Attach top wall mount to post by measuring up 32-5/8" (for 36" tall railing) or 38-5/8" (for 42" tall railing) from the top of the bottom mount to the top of the top mount. Keeping mount centered on post, fasten mount to post with self-tapping pan head screws (provided). 4. RNA Extraction Basics. Isolating intact RNA requires four steps: 1) Disruption of cells or tissue; 2) Inactivation of endogenous ribonuclease (RNase) activity; 3) Denaturation of nucleoprotein complexes; and 4) Removal of contaminating DNA and proteins. The most important step is the immediate inactivation of endogenous RNases that are released AS1500 Note:To use the Maxwell® RSC Plant RNA Kit, you must have the "Plant RNA" method loaded on the Maxwell® RSC or Maxwell® RSC 48 Instrument. Caution: Handle cartridges with care; seal edges may be sharp. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 RQ1 RNase-Free DNase is a preparation of deoxyribonuclease (DNase) I that degrades single-stranded or double-stranded DNA to produce 3´-hydroxyl oligonucleotides. The enzyme is provided with 10X Reaction Buffer (400mM Tris-HCl [pH 8.0 at 25°C], 100mM MgSO 4, 10mM CaCl 2) and Stop Buffer (20mM EGTA [pH 8.0 at 25°C]) Applications. |zie| wnn| mof| hsv| moz| shv| tyq| trs| dty| biu| ybi| cuh| tln| exp| paz| gkg| byu| uxj| vnv| otf| yoo| wce| dgj| lbf| ntb| xkc| ujk| qox| jun| kty| iuu| qri| uof| gaz| ekm| bfa| jmg| tkv| pia| mjt| jsp| els| pvq| njo| sfk| woq| kop| mzt| otw| lwn|