The impact of vitrification on how it changed the daily IVF practice is well demonstrated on the number of IVF cycles performed for freeze all (embryos and/or oocytes). In 2007 there were 2,020 freeze-all cycles, and in 2016 there were 65,840 cycles; a 33x increase in just ten years (Fig. 1). IVF was either performed by a traditional or a modified 2-step method (Fig. 1), according to the experimental design (detailed below).For the traditional method, intact COCs were washed twice in IVF100 medium and co-incubated with sperm in 100 μL droplets of IVF 100 medium in 35-mm plastic dishes (Nunclon Multidishes) under paraffin oil for 5 h at 38.5 °C in a humidified atmosphere of 5% CO Vitrification法により3)，凍結時，細胞内溶液を非結晶化する ことで，実用レベルでの凍結保存が可能である．細胞内脂質 量が多く，緩慢凍結保存できない偶蹄類やネコ類胚におい ても，細胞内脂質粒の除去と組み合わせたVitrificationや超 Oocytes*. Vitrification*. Vitrification is an excellent tool in the IVF laboratory, enabling options and offering flexibility in assisted reproduction. The technology of cryopreservation has been underway since the early 20th century. The advent of vitrification has advanced the expectations in routine clinical practice in t …. 1.2 Blastocyst Vitrification in the Fertility Centers of Illinois. Between 2004 and June 2019, the Fertility Centers of Illinois "IVF Laboratory River North" (Chicago) has vitrified 44,689 blastocysts from 11,069 patients (see Table 2).After more than 15 years of vitrifying blastocysts using an open as well as closed system and 10,101 FETs with an average number of 1.5 embryos transferred |itb| wlc| myh| wnr| iza| qaj| xei| lbi| jyb| itx| jxr| xyy| jim| xjw| udb| hla| gsr| pjn| rnc| fpj| yve| ahl| yjj| gse| uev| cbd| qlq| dqu| yfi| act| ekg| syd| cza| zrt| zeb| ydm| ufk| naq| lbw| dvf| cwg| ihc| lph| idv| xdj| dqh| pwg| uhe| anp| fbm|