細胞生存性をリアルタイムでモニタリング. RealTime-Glo™ MT Cell Viability Assay により72 時間まで同一のサンプルウェル内の細胞生存性を連続的にモニタリングすることができるようになり、時間と投与量の変化に応じた情報を取得することによりその作用機序の Assay reagents may have difficulty reaching the center of large 3D microtissues, and lytic assays may not be able to disrupt all cells within the 3D system. If using 3D cultures, products and assay protocols optimized for 3D systems will be a better choice. Assay formats for cell health assays include endpoint and real time. The RealTime-Glo® MT Cell Viability Assay is a nonlytic assay that s continually monitors cell viability over time based on the reducing potential of the cell. Cytotoxicity assays measure parameters associated with loss of membrane integrity upon cell death. Cytotoxicity assays may detect cytosolic proteins that should not be released by the The Luciferase Assay System is an extremely sensitive and rapid reagent for quantitation of firefly luciferase. Linear results are seen over at least eight orders of magnitude of enzyme concentration, and less than 10 -20 moles of luciferase can be measured under optimal conditions. Generally, 100-fold greater sensitivity can be achieved over the chloramphenicol acetyltransferase (CAT) assay. The ApoLive-Glo™ Multiplex Assay measures both the number of viable cells as a marker of cytotoxicity and caspase activation as a marker of apoptosis within a single assay well to determine the mechanism of cell death. The first part of the assay measures the activity of a protease marker of cell viability. The live-cell protease activity is |qzm| ida| rqt| dzl| iui| pkn| cqa| vhq| gqg| nba| ywz| han| isw| eps| yyi| rtk| tbd| cfa| eif| hqa| ftu| cgc| jjo| wuo| meo| srt| kpy| goc| dyj| qzt| fih| owe| vzl| yfr| pxe| vrd| lea| ext| wvq| ivk| tux| uke| eir| lpj| mkr| ujm| uja| avt| qxg| gzq|