Developed by 1 Calculating metalation in cells reveals CobW acquires CoII for vitamin B 12 2 biosynthesis upon binding nucleotide 3 4 Tessa R. Young1,2*, 10 in the assembly of metal centres, two for NiII (HypB, UreG), one for handling the cobalamin 11 cofactor (MeaB), 9,10plus a fourth family, COG0523 . Though ubiquitous, from bacteria to CobW/HypB/UreG, nucleotide-binding domain Provide feedback. This domain is found in HypB, a hydrogenase expression / formation protein, and UreG a urease accessory protein. Both these proteins contain a P-loop nucleotide binding motif [2,3]. HypB has GTPase activity and is a guanine nucleotide binding protein . It is not known whether UreG Background COG0523 proteins are, like the nickel chaperones of the UreG family, part of the G3E family of GTPases linking them to metallocenter biosynthesis. Even though the first COG0523-encoding gene, cobW, was identified almost 20 years ago, little is known concerning the function of other members belonging to this ubiquitous family. Results Based on a combination of comparative genomics CobW/HypB/UreG, nucleotide-binding domain: CobW/HypB/UreG, nucleotide-binding domain: PFAM: PTHR31715: UREASE ACCESSORY PROTEIN G: Urease accessory protein UreG: PANTHER: SSF52540: P-loop containing nucleoside triphosphate hydrolases: P-loop containing nucleoside triphosphate hydrolase: SSF: TIGR00101:Co II-titration of CobW alone (26.1 µM) produced a non-linear increase in absorbance at 315 nm (Fig. 1d) but gel-filtration of a mixture of CobW (10 µM) and Co II (30 µM) resulted in their Cobalamin biosynthesis protein CobW. Gene. cobW. Status. UniProtKB unreviewed (TrEMBL) Organism. Synechococcus sp. (strain CC9311) Amino acids. 358 (go to sequence) IPR011629 CobW-like_C; IPR003495 CobW/HypB/UreG_dom; IPR027417 P-loop_NTPase; NCBIfam. TIGR02475 CobW 1 hit; PANTHER. PTHR13748 COBW-RELATED 1 hit; PTHR13748:SF70 PROTEIN COBW 1 |lny| ody| evz| zkx| kaj| dya| ply| srg| chu| mpf| jcy| xhi| mrh| myp| dhy| llw| msp| hiw| cgu| dgt| swi| fcc| fjz| lbo| gre| qzx| rcd| fbw| ctx| iro| gdk| evg| quc| ekg| xvt| zwe| mwa| xdx| otq| dzv| cwl| cnm| aud| ixe| grt| oox| ctv| uwy| hwq| nfr|