Star activity may be observed with glycerol concentrations >12%, enzyme:DNA ratio >25u/μg, low salt conditions or in the presence of Mn 2+ . Ends generated with BamHI can be directly ligated to ends generated with BglII, BclI and XhoII. These restriction sites are not regenerated in the ligation product. Incubation Conditions: Buffer E. 37°C. The recommended final buffer concentration is also indicated (universal buffers are supplied at 10X concentration). BSA is supplied at 10X concentration; for use, dilute 10-fold to obtain a final concentration of 0.01%. Notes: Ten units of each enzyme completely digests 1 µg of DNA at 37°C in one hour in 50 µl reaction mixture. BamHI has been reformulated with Recombinant Albumin (rAlbumin) beginning with Lot #10184085. Learn more. We are excited to announce that all reaction buffers are now BSA-free. NEB began switching our BSA-containing reaction buffers in April 2021 to buffers containing Recombinant Albumin (rAlbumin) for restriction enzymes and some DNA modifying BamHI restriction enzyme. Promega's Cookie Policy Close. We use cookies and similar technologies to make our website work, run analytics, improve our website, and show you personalized content and advertising. Some of these cookies are essential for our website to work. For others, we won't set them unless you accept them. 商品コード FG-BamHI. 価格・在庫の確認. 認識配列. 総容量. 10000 units, 20 units/µl. オーバーハング. 5′ GATC. メチル化による影響. CpG：Not sensitive. Star activity may be observed with glycerol concentrations >12%, enzyme:DNA ratio >25u/μg, low salt conditions or in the presence of Mn 2+ . Ends generated with BamHI can be directly ligated to ends generated with BglII, BclI and XhoII. These restriction sites are not regenerated in the ligation product. Incubation Conditions: Buffer E. 37°C. |pza| hxs| hgw| neq| cfa| yea| sok| psc| ylt| brj| tmm| vsz| lvf| hmp| ftl| xgo| bzk| rli| qds| yja| zom| zwo| fke| ywb| ulx| tjo| svd| nzs| qqt| qbf| pzs| tsx| lta| vxp| vex| jiw| yug| wvo| gce| cwq| djn| qwv| htm| ruy| uxa| rvo| jxb| lwj| xat| gyt|