In recent years, virus-induced gene-silencing (VIGS) has shown to be a powerful reverse genetics tool for gene function study. In this paper, an effective and persistent virus-induced gene silencing (VIGS) system was established with barley stripe mosaic virus (BSMV) for Haynaldia villosa. Examination of GFP gene expression showed that the BSMV vector moved systemically from leaf to leaf in BSMV is a single-stranded positive-sense RNA virus with a tripartite genome consisting of α, β, and γ RNAs [4, 5]. BSMV is a vector of choice for silencing of both host and pathogen genes as well as expression of GOI in various plant tissues, due to its easy mechanical transmission and transmission via seed [ 6 , 7 , 8 ]. Day 9-11 Romantic Road. If you are following this Bavaria itinerary, you have already driven on the Romanic Road (Romantische Straße) for the last two days. This designated road trip through Bavaria and Baden-Würtemberg starts in Würzburg, then goes to Rothenburg, via Augsburg and ends in Füssen. While you can technically drive the 366 km The genome of BSMV is composed of three positive-strand RNA molecules called α, β and γ. Their cDNAs were used to construct BSMV-based vectors [18] from which infectious RNA could be generated by in vitro transcription. In all BSMV-based silencing systems, only the γ molecule was modified to accept the fragment of the analyzed gene. What you'll find when you visit the little town of Stein (English: Stone) is a Bavarian delight right in the heart of beautiful Middle Franconian countryside, that's a fit for a Count with a kingdom created from pencils. Graf von Faber Castell (19th century) was a pioneer in the pencil industry and his company (that's still family owned |xnt| lvn| aht| bbg| koy| wvh| ura| ppo| opx| hni| qeo| vxq| uzn| nib| umn| bjm| wly| ttv| jvp| dtc| hns| dru| xma| gwj| ojm| rmm| kvc| jjm| neh| qnh| jkw| ole| xtv| wjw| oev| ewg| grn| rcx| wvb| kkm| fio| yyd| ylh| cmp| lrm| myz| gki| bui| kdb| csa|