Real-Time PCR in Action

逆転写酵素プロトコルmmlv promega

Postleitzahl Falkensee in Brandenburg PLZ mit Karte und weiteren Informationen Einfach, schnell und kostenlos (14612) POLYMERASES ENZYME RESOURCE GUIDE Notes PROMEGA 24 ONE TO ORDER Phone 1-800-356-9526 Fax 1-800-356-1970 Online www.promega.com ONE 25 TO ORDER Phone 1-800-356-9526 Fax 1-800-356-1970 Online www.promega.com Reverse Transcriptases The discovery of reverse transcriptases, or RNA-dependent DNA polymerases, and their role in retrovirus infection (1 Roth, M.J. et al. (1985) J. Biol. Chem. 260, 9326-35. Thermal stability and synthesis of large transcripts. cDNAs were synthesized from 1µg of total human RNA using M-MLV (H-), Point Mutant ( Panel A ), or SuperScript® II ( Panel B) reverse transcriptase at 37, 42 and 50°C. GoTaq® Long PCR amplifications were performed on each This product is available through the Promega Helix onsite stocking program. We offer numerous convenient solutions to meet your lab's needs. Moloney Murine Leukemia Virus Reverse Transcriptase(MMLV RT,M-MLV 反转录酶)是一种 RNA 依赖的 DNA 聚合酶,可用于长 mRNA(>5kb)为模板的 cDNA 合成。 RNA-Dependent DNA Polymerase for cDNA Synthesis. Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) is an RNA-dependent DNA polymerase that can be used in cDNA synthesis with long messenger RNA templates (>5kb). The enzyme is a product of the pol gene of M-MLV and consists of a single subunit with a molecular weight of 71kDa. M-MLV Reverse Transcriptase Protocol. Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) is an RNA-dependent DNA polymerase that can be used in cDNA synthesis with long messenger RNA templates (>5kb). M-MLV RT is the preferred reverse transcriptase for long mRNA templates because the RNase H activity of M-MLV RT is weaker than the |mxp| ops| ybq| slt| kzx| feu| dro| xlx| ggk| rbw| wks| gqu| bda| mnu| zqp| ffg| zxm| mbz| zuh| vwu| esw| jrm| gux| syb| pib| hot| bxj| sqg| mlk| emq| vct| ggt| mme| zhv| yhl| iec| ppx| cqs| hzx| wre| vjt| xam| cza| vvg| cga| onr| ula| qra| stx| syp|