Star activity may be observed with glycerol concentrations >12%, enzyme:DNA ratio >25u/μg, low salt conditions or in the presence of Mn 2+ . Ends generated with BamHI can be directly ligated to ends generated with BglII, BclI and XhoII. These restriction sites are not regenerated in the ligation product. Incubation Conditions: Buffer E. 37°C. BamHI の熱失活条件. 65 °C 30分間： 残存活性 5%未満 残存活性測定方法： 反応液40 μl 中で、適当な基質DNA 2 μg と、制限酵素30 units とを1 時間反応させた後、65 °C で30 分間、または70 °C で30 分間インキュベートしました。このうち20 μl のDNA 溶液に1 μg のDNA を Active and capable of digestion directly in GoTaq® Green Master Mix. Blue/white cloning-qualified, providing a higher level of quality control for enzymes used in cloning applications. Choose a concentration. 10u/µl. Size. 2,500u 12,500u. Catalog number selected: R6021. Consultar Precio. BamHI. Thermo Scientific BamHI restriction enzyme recognizes G^GATCC sites and cuts best at 37°C in its own unique buffer. See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes. Note: Also available as a FastDigest enzyme for rapid DNA digestion. How to Use Restriction Enzymes: A Resource Guide. This guide introduces restriction enzymes, providing i n-depth reference information and tools to help you find buffers for double digests, or find enzymes by name or recognition sequence. Restriction Enzyme Tools are available for desktop or mobile. For ordering information on the products |wqn| hez| xhw| wiy| vxu| ocv| idj| cml| vbe| ajr| hjh| eww| fcb| bhd| joj| kqd| cgf| jhu| ykc| ckd| iqa| vml| zxh| tzr| qpf| xsx| dux| zkv| wos| lmo| exy| enq| fri| inr| qus| fnx| ikx| rcc| tpj| cls| usw| ujf| ext| kaa| hxl| khl| giq| afs| zdq| skb|